> For the complete documentation index, see [llms.txt](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/1-workshop-building-a-reagent-collaborative-network-in-latin-america.md).

# #1 WORKSHOP Building a Reagent Collaborative Network in Latin America

Welcome to the workshop: “Building a Reagent Collaborative Network in Latin America”, taking place from June 2 to 4, 2025, to promote open science through the collaborative development

This workshop is organized by the **Reclone LATAM Hub** at the **Universidad Nacional de Cuyo**, led by **María Teresa Damiani** (Reclone Hub LATAM Lead), **Mariángeles Ávila Maniero**, **Antonella Losinno**, and **Vanesa Gaona** (Reclone Hub LATAM Team); in collaboration with **Fernan Federici** (Reclone cofounder & Chile Node Lead), **Severine Cazaux**, and **Valentina Ferrando** (Reclone Chile Node Team).

**June 2** will feature theoretical sessions available in a **virtual format**.\
**June 3 and 4** will include **in-person hands-on activities** in laboratories at the **Universidad Nacional de Cuyo, Mendoza, Argentina**.

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### 📌 <mark style="color:red;">Registration</mark>

To participate in the workshop, please complete the following registration form:\
🔗 <https://forms.gle/DSvNseG4qD7K8HF46>

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### <mark style="color:red;">📅 Day 1 – Monday, June 2</mark>&#x20;

Theoretical session, Área de innovación UNCUYO

&#x20;(link for virtual attenders: <https://us02web.zoom.us/j/85686806331?pwd=498DPllpSlH4wlMctHK8LNFsdvcUzy.1>)&#x20;

**Focus:** Introduction to the RECLONE network and regional experiences

<table data-header-hidden><thead><tr><th width="168"></th><th></th></tr></thead><tbody><tr><td>10:00 – 10:30</td><td><em><strong>Welcome and opening remarks</strong></em> </td></tr><tr><td></td><td><p>Rector Esther Sánchez - Authorities of the Universidad Nacional de Cuyo – </p><p>Local organizers</p></td></tr><tr><td>10:30 – 11:00</td><td><em><strong>Presentation of RECLONE and the Latin American HUB (Mendoza, AR)</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub Lead Maria Teresa Damiani</td></tr><tr><td>11:00 – 11:30</td><td><em><strong>Previous RECLONE experiences in Latin America</strong></em></td></tr><tr><td></td><td>Reclone cofounder and Chile Node Lead Fernan Federici</td></tr><tr><td>11:30 – 12:00</td><td><em><strong>Community, events, and networking</strong></em></td></tr><tr><td></td><td>Latam Reclone community manager Cibele Zolnier</td></tr><tr><td>12:00 – 12:30</td><td><em><strong>Open Access materials, OpenMTA, and request procedures</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub researcher Mariángeles Ávila </td></tr><tr><td>13:00 - 14:00  </td><td><strong>Lunch</strong></td></tr><tr><td>14:00 – 17:00</td><td><em><strong>Participant presentations (3 minutes each)</strong></em></td></tr><tr><td></td><td>All participants</td></tr></tbody></table>

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### <mark style="color:red;">📅 Day 2 – Tuesday, June 3</mark>&#x20;

Hands-on practical session, IBYB-FCM-UNCUYO

**Focus:** Technical training and collaborative lab work

<table data-header-hidden><thead><tr><th width="165"></th><th></th></tr></thead><tbody><tr><td>10:00 – 10:30</td><td><em><strong>Group formation and protocol distribution</strong></em> </td></tr><tr><td></td><td>Reclone Latam Hub researcher Antonella Losinno</td></tr><tr><td>10:30 – 11:00</td><td><em><strong>Plasmid kit distribution</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician Vanessa Gaona</td></tr><tr><td>11:00 – 13:00</td><td><em><strong>Plasmid handling (resuspension from ethanol or filter paper)</strong></em></td></tr><tr><td></td><td><p>Vanessa Gaona</p><p>Severine Cazaux</p><p>Valentina Ferrando<br>Reclone Latam Team</p></td></tr><tr><td>13:00 - 14:00  </td><td><strong>Lunch</strong></td></tr><tr><td>14:00 – 17:00</td><td><em><strong>Bacterial transformation</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician Vanessa Gaona</td></tr></tbody></table>

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### <mark style="color:red;">📅 Day 3 – Wednesday, June 4</mark>&#x20;

Hands-on practical session, IBYB-FCM-UNCUYO

&#x20;Results analysis, reagent production, and closing

<table data-header-hidden><thead><tr><th width="160"></th><th></th></tr></thead><tbody><tr><td>10:00 – 12:30</td><td><em><strong>PCR confirmation of successful transformations</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician Vanessa Gaona</td></tr><tr><td>12:30 – 14:00</td><td><strong>Lunch</strong></td></tr><tr><td>14:00 – 16:00</td><td><em><strong>Cellular Reagent preparation (initial processing: OD600, dilution, inactivation, drying)</strong></em></td></tr><tr><td></td><td><p>Vanessa Gaona</p><p>Severine Cazaux</p><p>Valentina Ferrando<br>Reclone Latam Team</p></td></tr><tr><td>16:00 – 17:00</td><td><em><strong>Open Q&#x26;A forum</strong></em></td></tr><tr><td></td><td>Mariángeles Ávila, Antonella Losinno, Vanessa Gaona <br>Reclone Latam Hub Team</td></tr><tr><td>17:00 – 18:00</td><td><em><strong>Closing session</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub Lead Maria Teresa Damiani</td></tr></tbody></table>

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### <mark style="color:red;">✏️ Notes:</mark>

* The workshop will be conducted in Spanish.
* Digital protocols will be provided by QR code
* Each participant will sign an openMTA to receive a set of enzyme-expressing plasmids in filter paper or ethanol-precipitated format.

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### <mark style="color:red;">🧪 Plasmids and enzymes provided</mark>

You will find detailed information about the plasmids and enzymes that will be provided during the workshop.

Please note that the plasmids will be delivered either **precipitated** or stored on **filter paper** to ensure their stability during transport and storage. The enzymes included in these plasmids are essential for molecular biology techniques covered in the workshop.

***

The enzymes encoded in the delivered plasmids have been **previously purified following the** [**protocol**](https://dx.doi.org/10.17504/protocols.io.kqdg3wz8ev25/v2)**.**&#x54;his method describes the batch purification of recombinant His-tagged proteins expressed in *E. coli* using **Ni-NTA (Nickel-Nitrilotriacetic Acid) beads.**

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<table><thead><tr><th width="45">No.</th><th width="114">Enzyme</th><th width="140">Resistance Marker</th><th width="128">Vector</th><th width="137">Plasmid Size (bp)</th><th></th></tr></thead><tbody><tr><td>1</td><td><a href="https://dx.doi.org/10.17504/protocols.io.bksrkwd6">Bst mCherry</a></td><td>Kanamycin</td><td>pTi</td><td></td><td></td></tr><tr><td>2</td><td><a href="https://dx.doi.org/10.17504/protocols.io.j8nlkk1xwl5r/v2">Open Vent mCherry</a></td><td>Kanamycin</td><td>pTi</td><td>7974</td><td>6xHis</td></tr><tr><td>3</td><td>Pfu DNA polymerase</td><td>Kanamycin</td><td>pET-28a(+)</td><td>7800</td><td>10xHis</td></tr><tr><td>4</td><td><a href="https://dx.doi.org/10.17504/protocols.io.bsejnbcn">MMLV RT RNAse free</a></td><td>Ampicillin</td><td>pTi</td><td>7187</td><td>6xHis</td></tr><tr><td>5</td><td>Taq Polymerase (common)</td><td>Kanamycin</td><td>pET-28a(+)</td><td>7826</td><td>6xHis</td></tr></tbody></table>

***