📅#1 WORKSHOP Building a Reagent Collaborative Network in Latin America
Welcome to the workshop: “Building a Reagent Collaborative Network in Latin America”, taking place from June 2 to 4, 2025, to promote open science through the collaborative development
This workshop is organized by the Reclone LATAM Hub at the Universidad Nacional de Cuyo, led by María Teresa Damiani (Reclone Hub LATAM Lead), Mariángeles Ávila Maniero, Antonella Losinno, and Vanesa Gaona (Reclone Hub LATAM Team); in collaboration with Fernan Federici (Reclone cofounder & Chile Node Lead), Severine Cazaux, and Valentina Ferrando (Reclone Chile Node Team).
June 2 will feature theoretical sessions available in a virtual format. June 3 and 4 will include in-person hands-on activities in laboratories at the Universidad Nacional de Cuyo, Mendoza, Argentina.
📌 Registration
To participate in the workshop, please complete the following registration form: 🔗 https://forms.gle/DSvNseG4qD7K8HF46
📅 Day 1 – Monday, June 2
Theoretical session, Área de innovación UNCUYO
(link for virtual attenders: https://us02web.zoom.us/j/85686806331?pwd=498DPllpSlH4wlMctHK8LNFsdvcUzy.1)
Focus: Introduction to the RECLONE network and regional experiences
10:00 – 10:30
Welcome and opening remarks
Rector Esther Sánchez - Authorities of the Universidad Nacional de Cuyo –
Local organizers
10:30 – 11:00
Presentation of RECLONE and the Latin American HUB (Mendoza, AR)
Reclone Latam Hub Lead Maria Teresa Damiani
11:00 – 11:30
Previous RECLONE experiences in Latin America
Reclone cofounder and Chile Node Lead Fernan Federici
11:30 – 12:00
Community, events, and networking
Latam Reclone community manager Cibele Zolnier
12:00 – 12:30
Open Access materials, OpenMTA, and request procedures
Reclone Latam Hub researcher Mariángeles Ávila
13:00 - 14:00
Lunch
14:00 – 17:00
Participant presentations (3 minutes each)
All participants
📅 Day 2 – Tuesday, June 3
Hands-on practical session, IBYB-FCM-UNCUYO
Focus: Technical training and collaborative lab work
10:00 – 10:30
Group formation and protocol distribution
Reclone Latam Hub researcher Antonella Losinno
10:30 – 11:00
Plasmid kit distribution
Reclone Latam Hub technician Vanessa Gaona
11:00 – 13:00
Plasmid handling (resuspension from ethanol or filter paper)
Vanessa Gaona
Severine Cazaux
Valentina Ferrando Reclone Latam Team
13:00 - 14:00
Lunch
14:00 – 17:00
Bacterial transformation
Reclone Latam Hub technician Vanessa Gaona
📅 Day 3 – Wednesday, June 4
Hands-on practical session, IBYB-FCM-UNCUYO
Results analysis, reagent production, and closing
10:00 – 12:30
PCR confirmation of successful transformations
Reclone Latam Hub technician Vanessa Gaona
12:30 – 14:00
Lunch
14:00 – 16:00
Cellular Reagent preparation (initial processing: OD600, dilution, inactivation, drying)
Vanessa Gaona
Severine Cazaux
Valentina Ferrando Reclone Latam Team
16:00 – 17:00
Open Q&A forum
Mariángeles Ávila, Antonella Losinno, Vanessa Gaona Reclone Latam Hub Team
17:00 – 18:00
Closing session
Reclone Latam Hub Lead Maria Teresa Damiani
✏️ Notes:
The workshop will be conducted in Spanish.
Digital protocols will be provided by QR code
Each participant will sign an openMTA to receive a set of enzyme-expressing plasmids in filter paper or ethanol-precipitated format.
🧪 Plasmids and enzymes provided
You will find detailed information about the plasmids and enzymes that will be provided during the workshop.
Please note that the plasmids will be delivered either precipitated or stored on filter paper to ensure their stability during transport and storage. The enzymes included in these plasmids are essential for molecular biology techniques covered in the workshop.
The enzymes encoded in the delivered plasmids have been previously purified following the protocol.This method describes the batch purification of recombinant His-tagged proteins expressed in E. coli using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads.
3
Pfu DNA polymerase
Kanamycin
pET-28a(+)
7800
10xHis
5
Taq Polymerase (common)
Kanamycin
pET-28a(+)
7826
6xHis
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