> For the complete documentation index, see [llms.txt](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/4-workshop-advanced-hands-on-science-for-university-educators.md).

# #4 Workshop: “Advanced Hands-on Science for University Educators”

Bienvenidos al tercer encuentro de la iniciativa Reclone LATAM que se realizará los días 29 y 30 de septiembre de 2025 en el Espacio de Innovación de la UNCUYO, dentro del Mendoza TIC

Open source hardware para PCR desde reactivos celulares

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### <mark style="color:red;">📅 Day 1 – Monday, September 29</mark>&#x20;

<table data-header-hidden><thead><tr><th width="169.99993896484375">Time</th><th>Activity</th></tr></thead><tbody><tr><td>9:30 - 10:00</td><td>Bienvenida (Mendoza)</td></tr><tr><td>10:00 - 11:00</td><td>Charla introductoria OSH y kit (FF)</td></tr><tr><td>11:00 - 13:00</td><td>Recolección y lavado de pellet / centrifugación</td></tr><tr><td>13:00 - 14:00</td><td>Almuerzo</td></tr><tr><td>14:00 - 16:00</td><td>Medición de OD y dilución de las células</td></tr><tr><td>16:00 - 18:00</td><td>Registro OD y fotografía fluorescencia</td></tr></tbody></table>

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### <mark style="color:red;">📅 Day 2 – Tuesday, September 30</mark>

<table data-header-hidden><thead><tr><th width="169.99993896484375">Time</th><th>Activity</th></tr></thead><tbody><tr><td>9:30 - 10:00</td><td>Charla Reactivos + OSH (FF, Sev)</td></tr><tr><td>10:00 - 11:00</td><td>PCR</td></tr><tr><td>11:00 - 13:00</td><td>Charlas OSH (FF, Nico)</td></tr><tr><td>13:00 - 14:00</td><td>Almuerzo </td></tr><tr><td>14:00 - 16:00</td><td>Electroforesis</td></tr><tr><td>16:00 - 18:00</td><td>Charlas OSH (FF, Wladi) y cierre </td></tr></tbody></table>

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### <mark style="color:red;">✏️ Notes:</mark>

* The workshop will be conducted in Spanish.
* Digital protocols will be provided by QR code
* Each participant will sign an openMTA to receive a set of enzyme-expressing plasmids in filter paper or ethanol-precipitated format.

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### <mark style="color:red;">🧪 Plasmids and enzymes provided</mark>

You will find detailed information about the plasmids and enzymes that will be provided during the workshop.

Please note that the plasmids will be delivered either **precipitated** or stored on **filter paper** to ensure their stability during transport and storage. The enzymes included in these plasmids are essential for molecular biology techniques covered in the workshop.

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The enzymes encoded in the delivered plasmids have been **previously purified following the** [**protocol**](https://dx.doi.org/10.17504/protocols.io.kqdg3wz8ev25/v2)**.**&#x54;his method describes the batch purification of recombinant His-tagged proteins expressed in *E. coli* using **Ni-NTA (Nickel-Nitrilotriacetic Acid) beads.**

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<table><thead><tr><th width="45">No.</th><th width="114">Enzyme</th><th width="74.1025390625">Resistance Marker</th><th width="128">Vector</th><th width="137">Plasmid Size (bp)</th><th></th></tr></thead><tbody><tr><td>1</td><td><a href="https://dx.doi.org/10.17504/protocols.io.bksrkwd6">Bst</a></td><td>Kanamycin</td><td>pTi</td><td>6970</td><td></td></tr><tr><td>2</td><td><a href="https://dx.doi.org/10.17504/protocols.io.j8nlkk1xwl5r/v2">Open Vent mCherry</a></td><td>Kanamycin</td><td>pTi</td><td>7974</td><td>6xHis</td></tr><tr><td>4</td><td><a href="https://dx.doi.org/10.17504/protocols.io.bsejnbcn">MMLV RT RNAse free</a></td><td>Ampicillin</td><td>pTi</td><td>7187</td><td>6xHis</td></tr></tbody></table>

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