📅#2 WORKSHOP Building a Reagent Collaborative Network in Latin America
Welcome to the #2 workshop: “Building a Reagent Collaborative Network”, taking place on July 31 and August 1, 2025, at the Espacio de INNOVACIÓN de la UNCUYO inside the Mendoza TIC parque tecnológico
This local edition is focused on promoting open science practices through hands-on training and the collaborative use of accessible biological reagents.
It is organized by the Reclone LATAM Hub Team at UNCUYO, coordinated by María Teresa Damiani, Mariángeles Ávila Maniero, Antonella Losinno, and Vanesa Gaona.
More details about the session schedule will follow below.
📅 Day 1 – Thursday, July 31
09:00 - 09:20
Open Science and Reclone LatinAmerican Hub at UNCUYO (Mendoza, AR)
Reclone Latam Hub Lead: Maria Teresa Damiani
09:20 - 09:30
Introduction to Reclone global
Reclone Co-Founder: Jenny Molloy (University of Cambridge, London, UK)
09:30 – 09:45
Previous RECLONE experiences in Latin America
Reclone cofounder and Chile Node Lead Fernan Federici
09:45 - 10:00
Reclone Perù Node Presentation
Reclone Perù Node Lead: Daniel Guerra
10:00 - 10:10
Community, events, and networking
Latam Reclone community manager Cibele Zolnier
10:10 - 10:30
Open Access materials, OpenMTA, and request procedures
Reclone Latam Hub researcher: Mariángeles Ávila
10:30 - 10:40
Team Presentation: Instituto de Bioquímica y Biotecnología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo
Protein Visualization and Resuspension from Cellular Reagent
Reclone Latam Hub technician Vanessa Gaona
09:30 - 12:30
PCR confirmation of successful transformations
Reclone Latam Hub technician Vanessa Gaona
10:00 - 11:30
Theoretical Overview: Purification of His-Tagged Proteins Using Ni-NTA Beads
Reclone Latam Hub technician: Vanessa Gaona
11:30 - 12:00
Networking: Coffee break
12:00 - 13:30
Lunch
13:30 – 14:30
Visualization of PCR Results by Gel Electrophoresis
Reclone Latam Hub technician Vanessa Gaona
14:30 – 15:00
Plasmid kit distribution
Reclone Latam Hub researchers: Mariángeles Ávila and Antonella Losinno
15:00 – 16:00
Closing session
Reclone Latam Hub Lead: Maria Teresa Damiani
✏️ Notes:
The workshop will be conducted in Spanish.
Digital protocols will be provided by QR code
Each participant will sign an openMTA to receive a set of enzyme-expressing plasmids in filter paper or ethanol-precipitated format.
🧪 Plasmids and enzymes provided
You will find detailed information about the plasmids and enzymes that will be provided during the workshop.
Please note that the plasmids will be delivered precipitated to ensure their stability during transport and storage. The enzymes included in these plasmids are essential for the molecular biology techniques discussed in the workshop.
The enzymes encoded in the delivered plasmids have been previously purified following the protocol.This method describes the batch purification of recombinant His-tagged proteins expressed in E. coli using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads.