> For the complete documentation index, see [llms.txt](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/2-workshop-building-a-reagent-collaborative-network-in-latin-america.md).

# #2 WORKSHOP Building a Reagent Collaborative Network in Latin America

Welcome to the #2 workshop: “Building a Reagent Collaborative Network”, taking place on July 31 and August 1, 2025, at the Espacio de INNOVACIÓN de la UNCUYO inside the Mendoza TIC parque tecnológico

This local edition is focused on promoting **open science practices** through hands-on training and the collaborative use of **accessible biological reagents**.\
It is organized by the **Reclone LATAM Hub Team at UNCUYO**, coordinated by **María Teresa Damiani, Mariángeles Ávila Maniero, Antonella Losinno, and Vanesa Gaona**.

More details about the session schedule will follow below.

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### <mark style="color:red;">📅 Day 1 – Thursday, July 31</mark>

<table data-header-hidden><thead><tr><th width="168"></th><th></th></tr></thead><tbody><tr><td>09:00 - 09:20</td><td><em><strong>Open Science and Reclone LatinAmerican Hub at UNCUYO (Mendoza, AR)</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub Lead: Maria Teresa Damiani</td></tr><tr><td>09:20 - 09:30</td><td><em><strong>Introduction to Reclone global</strong></em></td></tr><tr><td></td><td>Reclone Co-Founder: Jenny Molloy (University of Cambridge, London, UK)</td></tr><tr><td>09:30 – 09:45</td><td><em><strong>Previous RECLONE experiences in Latin America</strong></em></td></tr><tr><td></td><td>Reclone cofounder and Chile Node Lead Fernan Federici</td></tr><tr><td>09:45 - 10:00</td><td><strong>Reclone Perù Node Presentation</strong></td></tr><tr><td></td><td>Reclone Perù Node Lead: Daniel Guerra</td></tr><tr><td>10:00 - 10:10</td><td><em><strong>Community, events, and networking</strong></em></td></tr><tr><td></td><td>Latam Reclone community manager Cibele Zolnier</td></tr><tr><td>10:10 - 10:30</td><td><em><strong>Open Access materials, OpenMTA, and request procedures</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub researcher: Mariángeles Ávila</td></tr><tr><td>10:30 - 10:40</td><td><em><strong>Team Presentation: Instituto de Bioquímica y Biotecnología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub researcher: Antonella Losinno</td></tr><tr><td></td><td><em><strong>Participant presentations (3 minutes each)</strong></em></td></tr><tr><td>10:40 - 13:00</td><td>All participants (see <a href="https://docs.google.com/presentation/d/1WCXvyf01GDLkiX-HHNG2kId7-FKa99M_qet4fo2PiAo/edit?usp=sharing">template</a>)</td></tr><tr><td>13:00 - 14:00  </td><td><em><strong>Networking (coffee break) and lunch</strong></em></td></tr><tr><td>14:00 - 14:30</td><td><em><strong>Plasmid handling (resuspension from ethanol precipitation)</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician: Vanessa Gaona</td></tr><tr><td>14:30 - 17:00</td><td><em><strong>Bacterial transformation</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician: Vanessa Gaona</td></tr><tr><td>15:30 - 16:30</td><td><em><strong>Cellular Reagent preparation (initial processing: OD600, dilution, inactivation, drying)</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician: Vanessa Gaona</td></tr></tbody></table>

***

### <mark style="color:red;">📅 Day 2 – Friday, August 1</mark>&#x20;

<table data-header-hidden><thead><tr><th width="165"></th><th></th></tr></thead><tbody><tr><td>09:00 - 09:30</td><td><em><strong>Protein Visualization and Resuspension from Cellular Reagent</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician Vanessa Gaona</td></tr><tr><td>09:30 - 12:30</td><td><em><strong>PCR confirmation of successful transformations</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician Vanessa Gaona</td></tr><tr><td>10:00 - 11:30</td><td><em><strong>Theoretical Overview: Purification of His-Tagged Proteins Using Ni-NTA Beads</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician: Vanessa Gaona</td></tr><tr><td>11:30 - 12:00 </td><td><em><strong>Networking: Coffee break</strong></em></td></tr><tr><td>12:00 - 13:30</td><td><em><strong>Lunch</strong></em></td></tr><tr><td>13:30 – 14:30</td><td><em><strong>Visualization of PCR Results by Gel Electrophoresis</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub technician Vanessa Gaona</td></tr><tr><td>14:30 – 15:00</td><td><em><strong>Plasmid kit distribution</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub researchers: Mariángeles Ávila and Antonella Losinno</td></tr><tr><td>15:00 – 16:00</td><td><em><strong>Closing session</strong></em></td></tr><tr><td></td><td>Reclone Latam Hub Lead: Maria Teresa Damiani</td></tr></tbody></table>

***

### <mark style="color:red;">✏️ Notes:</mark>

* The workshop will be conducted in Spanish.
* Digital protocols will be provided by QR code
* Each participant will sign an openMTA to receive a set of enzyme-expressing plasmids in filter paper or ethanol-precipitated format.

***

### <mark style="color:red;">🧪 Plasmids and enzymes provided</mark>

You will find detailed information about the plasmids and enzymes that will be provided during the workshop.

Please note that the plasmids will be delivered precipitated to ensure their stability during transport and storage. The enzymes included in these plasmids are essential for the molecular biology techniques discussed in the workshop.

***

The enzymes encoded in the delivered plasmids have been **previously purified following the** [**protocol**](https://dx.doi.org/10.17504/protocols.io.kqdg3wz8ev25/v2)**.**&#x54;his method describes the batch purification of recombinant His-tagged proteins expressed in *E. coli* using **Ni-NTA (Nickel-Nitrilotriacetic Acid) beads.**

***

<table><thead><tr><th width="45">No.</th><th width="114">Enzyme</th><th width="140">Resistance Marker</th><th width="128">Vector</th><th width="137">Plasmid Size (bp)</th><th></th></tr></thead><tbody><tr><td>1</td><td><a href="https://dx.doi.org/10.17504/protocols.io.bsejnbcn">Bst Lf</a></td><td>Kanamycin</td><td>pTi</td><td>6971</td><td>6xHis</td></tr><tr><td>2</td><td><a href="https://dx.doi.org/10.17504/protocols.io.j8nlkk1xwl5r/v2">Open Vent mCherry</a></td><td>Kanamycin</td><td>pTi</td><td>7974</td><td>6xHis</td></tr><tr><td>4</td><td><a href="https://dx.doi.org/10.17504/protocols.io.bsejnbcn">MMLV RT RNAse free</a></td><td>Ampicillin</td><td>pTi</td><td>7187</td><td>6xHis</td></tr></tbody></table>

***