{"version":1,"pages":[{"id":"wZxIbCZG1GZ8c96JUHkC","title":"RECLONE LATAM - UNCUYO","pathname":"/building-a-reagent-collaborative-network-in-latam","siteSpaceId":"sitesp_D1Gpp","emoji":"1f9ec","description":"Reclone LATAM – UNCUYO is part of the global RECLONE network, a collaborative community dedicated to the development of open and accessible biomedical technologies.","breadcrumbs":[{"label":"Welcome & Overview"}]},{"id":"l4pAOqOEl45pJkCqNjik","title":"NAVIGATION GUIDE","pathname":"/building-a-reagent-collaborative-network-in-latam/navigation-guide","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4bb"},{"id":"muqPh9fUIHCypZ0uWM1X","title":"#1 WORKSHOP Building a Reagent Collaborative Network in Latin America","pathname":"/building-a-reagent-collaborative-network-in-latam/1-workshop-building-a-reagent-collaborative-network-in-latin-america","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4c5","description":"Welcome to the workshop: “Building a Reagent Collaborative Network in Latin America”, taking place from June 2 to 4, 2025, to promote open science through the collaborative development"},{"id":"5evvONAaM6EDES3WPNre","title":"#2 WORKSHOP Building a Reagent Collaborative Network in Latin America","pathname":"/building-a-reagent-collaborative-network-in-latam/2-workshop-building-a-reagent-collaborative-network-in-latin-america","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4c5","description":"Welcome to the #2 workshop: “Building a Reagent Collaborative Network”, taking place on July 31 and August 1, 2025, at the Espacio de INNOVACIÓN de la UNCUYO inside the Mendoza TIC parque tecnológico"},{"id":"Hbo8wrNoEdPlYqB84pmB","title":"#3 WORKSHOP: Hands-on Science for High School Educators","pathname":"/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4c5","description":"Bienvenidos al tercer encuentro de la iniciativa RECLONE LATAM que se realizará los días 25 y 26 de septiembre de 2025 en el Espacio de Innovación de la UNCUYO, dentro del Mendoza TIC"},{"id":"9jQ5AZ7bSAEmEsavZ7Bi","title":"Material de apoyo y conceptos clave","pathname":"/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/material-de-apoyo-y-conceptos-clave","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4d3","breadcrumbs":[{"label":"#3 WORKSHOP: Hands-on Science for High School Educators","emoji":"1f4c5"}]},{"id":"AJCAgvul4c747Kw4Bft0","title":"Acceso al práctico","pathname":"/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/acceso-al-practico","siteSpaceId":"sitesp_D1Gpp","emoji":"1f5c2","description":"El documento completo del práctico “Hands-on Science for High School Educators – Sabor amargo” se encuentra disponible en línea.","breadcrumbs":[{"label":"#3 WORKSHOP: Hands-on Science for High School Educators","emoji":"1f4c5"}]},{"id":"85u4TI74SJJALICtQDsq","title":"Protocolos de laboratorio","pathname":"/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/protocolos-de-laboratorio","siteSpaceId":"sitesp_D1Gpp","emoji":"1f9ec","breadcrumbs":[{"label":"#3 WORKSHOP: Hands-on Science for High School Educators","emoji":"1f4c5"}]},{"id":"qKHvXzeRq8rcsySM7YZi","title":"Entregables","pathname":"/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/entregables","siteSpaceId":"sitesp_D1Gpp","emoji":"1f9ea","description":"Como parte de este workshop, cada participante recibirá un tubo de cellular reagent preparado a partir de E. coli BL21 que expresan la enzima OpenVent-mCherry.","breadcrumbs":[{"label":"#3 WORKSHOP: Hands-on Science for High School Educators","emoji":"1f4c5"}]},{"id":"oBN6dArg6oggKqP0NFz3","title":"#4 Workshop: “Advanced Hands-on Science for University Educators”","pathname":"/building-a-reagent-collaborative-network-in-latam/4-workshop-advanced-hands-on-science-for-university-educators","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4c5","description":"Bienvenidos al tercer encuentro de la iniciativa Reclone LATAM que se realizará los días 29 y 30 de septiembre de 2025 en el Espacio de Innovación de la UNCUYO, dentro del Mendoza TIC"},{"id":"Df7zQJamwWzhsH2GKpW9","title":"PLASMID STORAGE","pathname":"/building-a-reagent-collaborative-network-in-latam/general-protocols/plasmid-storage","siteSpaceId":"sitesp_D1Gpp","emoji":"1f4e6","description":"This page describes two validated methods for plasmid preservation and shipment without the need for cold chain transport: drying on filter paper and ethanol precipitation.","breadcrumbs":[{"label":"GENERAL PROTOCOLS"}]},{"id":"6LoKj0bNRQ7k2YRSMdl9","title":"BACTERIAL TRANSFORMATION","pathname":"/building-a-reagent-collaborative-network-in-latam/bacterial-transformation","siteSpaceId":"sitesp_D1Gpp","emoji":"1f9a0","description":"This protocol describes the chemical transformation using the classic heat shock method. It is suitable for introducing plasmid DNA into competent cells prepared"},{"id":"UpzdwdWsG1JNMLvHNcGn","title":"COLONY PCR","pathname":"/building-a-reagent-collaborative-network-in-latam/colony-pcr","siteSpaceId":"sitesp_D1Gpp","emoji":"1f9eb","description":"This PCR protocol is designed to confirm successful transformation of plasmids. In this case, the plasmid carrying the open vent mCherry gene includes a T7 promoter and T7 terminator, making it ideal"},{"id":"rSfqWrnV8AJiKOsLyA7i","title":"PROTEIN PURIFICATION","pathname":"/building-a-reagent-collaborative-network-in-latam/protein-purification","siteSpaceId":"sitesp_D1Gpp","emoji":"2697","description":"This protocol describes the batch purification of recombinant His-tagged proteins expressed in E. coli. Proteins containing a 6xHis tag are purified using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads."},{"id":"dkjKAWGmMgNLOXxGSryH","title":"CELLULAR REAGENT","pathname":"/building-a-reagent-collaborative-network-in-latam/cellular-reagent","siteSpaceId":"sitesp_D1Gpp","emoji":"1f321","description":"This protocol describes the preparation of a cell-based reagent from bacterial biomass, suitable for use as a crude lysate in molecular biology applications"},{"id":"IQxtllO7MZXXHfw88wsh","title":"TEAM MEMBERS","pathname":"/building-a-reagent-collaborative-network-in-latam/reclone-latin-america-team/team-members","siteSpaceId":"sitesp_D1Gpp","emoji":"1f97c","description":"This section introduces the dedicated team building and supporting the Reclone Latin America network.","breadcrumbs":[{"label":"Reclone Latin America Team"}]}]}