# Building a Reagent Collaborative Network in Latin America

## Reclone LATAM - UNCUYO

- [RECLONE LATAM - UNCUYO](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/welcome-and-overview/reclone-latam-uncuyo.md): Reclone LATAM – UNCUYO is part of the global RECLONE network, a collaborative community dedicated to the development of open and accessible biomedical technologies.
- [NAVIGATION GUIDE](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/navigation-guide.md)
- [#1 WORKSHOP Building a Reagent Collaborative Network in Latin America](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/1-workshop-building-a-reagent-collaborative-network-in-latin-america.md): Welcome to the workshop: “Building a Reagent Collaborative Network in Latin America”, taking place from June 2 to 4, 2025, to promote open science through the collaborative development
- [#2 WORKSHOP Building a Reagent Collaborative Network in Latin America](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/2-workshop-building-a-reagent-collaborative-network-in-latin-america.md): Welcome to the #2 workshop: “Building a Reagent Collaborative Network”, taking place on July 31 and August 1, 2025, at the Espacio de INNOVACIÓN de la UNCUYO inside the Mendoza TIC parque tecnológico
- [#3 WORKSHOP: Hands-on Science for High School Educators](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators.md): Bienvenidos al tercer encuentro de la iniciativa RECLONE LATAM que se realizará los días 25 y 26 de septiembre de 2025 en el Espacio de Innovación de la UNCUYO, dentro del Mendoza TIC
- [Material de apoyo y conceptos clave](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/material-de-apoyo-y-conceptos-clave.md)
- [Acceso al práctico](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/acceso-al-practico.md): El documento completo del práctico “Hands-on Science for High School Educators – Sabor amargo” se encuentra disponible en línea.
- [Protocolos de laboratorio](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/protocolos-de-laboratorio.md)
- [Entregables](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/3-workshop-hands-on-science-for-high-school-educators/entregables.md): Como parte de este workshop, cada participante recibirá un tubo de cellular reagent preparado a partir de E. coli BL21 que expresan la enzima OpenVent-mCherry.
- [#4 Workshop: “Advanced Hands-on Science for University Educators”](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/4-workshop-advanced-hands-on-science-for-university-educators.md): Bienvenidos al tercer encuentro de la iniciativa Reclone LATAM que se realizará los días 29 y 30 de septiembre de 2025 en el Espacio de Innovación de la UNCUYO, dentro del Mendoza TIC
- [PLASMID STORAGE](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/general-protocols/plasmid-storage.md): This page describes two validated methods for plasmid preservation and shipment without the need for cold chain transport: drying on filter paper and ethanol precipitation.
- [BACTERIAL TRANSFORMATION](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/bacterial-transformation.md): This protocol describes the chemical transformation using the classic heat shock method. It is suitable for introducing plasmid DNA into competent cells prepared
- [COLONY PCR](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/colony-pcr.md): This PCR protocol is designed to confirm successful transformation of plasmids. In this case, the plasmid carrying the open vent mCherry gene includes a T7 promoter and T7 terminator, making it ideal
- [PROTEIN PURIFICATION](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/protein-purification.md): This protocol describes the batch purification of recombinant His-tagged proteins expressed in E. coli. Proteins containing a 6xHis tag are purified using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads.
- [CELLULAR REAGENT](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/cellular-reagent.md): This protocol describes the preparation of a cell-based reagent from bacterial biomass, suitable for use as a crude lysate in molecular biology applications
- [TEAM MEMBERS](https://reclone-latam-uncuyo.gitbook.io/building-a-reagent-collaborative-network-in-latam/reclone-latin-america-team/team-members.md): This section introduces the dedicated team building and supporting the Reclone Latin America network.